Sepharose sp column torrent

Ge healthcare also sells prepacked hiprep 20ml columns with deae, sp, q sepharose. Use a weak ion exchanger such as deae, cm or anx sepharose fast flow, if a strong ion exchanger substituted with q, s or sp does not give the required selectivity. Protocols and tips in protein purification the university of sheffield. Protein purification pouring and packing an agarose column. Sp sepharose has also assisted with advancing industrial. Pour the gel slurry into the column in one continuous motion.

Availability in different prepacked hiprep and hitrap column formats plus. Why my target protein is not eluted from a qsepharose column. Titrationcurveofdeaesepharosefastflow 8 71500964ag. What is the best way of packing sepharose cl6b matrix in a. Application sp sepharose is used in protein chromatography, ion exchange chromatography and cation exchange media. What is the best way of packing sepharose cl6b matrix in a column. Sepharose high sp sepharose high performance q sepharose high performance, and sp sepharose high performance, are strong ion exchangers. Sp sepharose fast flow, aqueous ethanol suspension, 45. The software supports nonlinear modeling techniques such as construction sequencing and time effects. Sp sepharose fast flow can also be packed in the other column platforms such as bpg and chromaflow. Use sepharose fast flow media for purification of proteins.

For processscale applications, ge recommends axichrom columns. Cm sepharose fast flow deae sepharose fast flow q sepharose. Q sepharose high performance and sp sepharose high. Supplied connector kit with hitrap column media properties sp sepharose high performance and q sepharose high performance are strong cation and strong anion exchangers respectively. Sp sepharose has been used to study inhibitory proteins and peptides from the rhizomes of zingiberaceae plants as well as to study the subunit heterogeneity and molecular evolution of soybean basic 7s globulin. Equipped with latest american aci 31814 and canadian csa a23. Ff, but i do not think that they are cost effective, better to pack such. Wash the gel with 510 gel volumes of distilled water on a glass filter. With newly introduced spresults and spreporter modules and powered by the advanced and flexible graphical interface of new spsection module for creating and modifying irregular sections, spcolumn v6. The functional groups are coupled to the matrix via chemically stable ether linkages. Hitrap sp hp, 1 ml and 5 ml hitrap q hp, 1 ml and 5 ml. The columns have an advanced design and are constructed from precision bore borosilicate glass.

Sp sepharose fast flow cation exchange chromatography. Instructions71500964ag ionexchangeresins cmsepharose. Q sepharose high performance sp sepharose high performance prepacked hiload and hitrap columns q sepharose high performance and sp sepharose high performance enjoy welldeserved reputations as highly successful anion and cation ion exchange media for purifying a wide range of biomolecules. Can one use sp sepharose rather than mono s for protein. Ion exchange chromatography columns and resins ge healthcare. A m e r s h a m b i o s c i e n c e s q sepharose fast flow. Both remain charged and maintain high capacity over broad ph ranges. Can one use sp sepharose rather than mono s for protein purification. Instructions71500964ag ionexchangeresins cmsepharosefastflow deaesepharosefastflow qsepharosefastflow spsepharosefastflow cm,deae. Sp sepharose fast flow is compatible with two of these lines, tricorn and xk columns. Use sepharose fast flow for capture or intermediate purification steps that require good resolution flows up to 300 cmh. Use for intermediate purification if column capacity is sufficient and no scaleup.

It has the stateofart sapfire 64bit solver to analyze extremely large and complex projects rapidly. A m e r s h a m b i o s c i e n c e s important user information please read these instructions carefully before using q sepharose fast flow media. Pdf q sepharose high performance sp sepharose high. To modify these instructions for columns of different dimensions, refer to appendix a. The protocol calls for sp sepharose and subsequently cm sepharose columns for purification of the protein.